Sallam A M, Hwang N H
Biorheology. 1984;21(6):783-97. doi: 10.3233/bir-1984-21605.
Various previous models used in studying red blood cell (RBC) hemolysis in turbulent shear flows are reviewed from a fluid dynamic point of view. The effect of turbulent shear stress (Reynolds shear stress, tau R) on RBC hemolysis is investigated utilizing a submerged axisymmetric jet flow field. A detailed survey of the flow field is made with a laser Doppler anemometer system to obtain contour maps of the mean velocity distributions, relative turbulence intensities, and tau R distributions in the field prior to conducting the experiment of sampling and analyzing the cells free-hemoglobin in the field. A new two-point sampling technique, developed in this laboratory, allows collections of RBC samples from selected locations in the flow field so that a relationship between the local shear stress level and the cell damage may be established. The threshold level of tau R responsible for incipient hemolysis is found to be approximately 400 Newtons per square meter (N/m2), below which a sublethal region of zero hemolysis is observed.
从流体动力学的角度对以往用于研究湍流剪切流中红细胞（RBC）溶血的各种模型进行了综述。利用淹没轴对称射流流场研究了湍流剪切应力（雷诺剪切应力，τR）对红细胞溶血的影响。在进行现场细胞游离血红蛋白的采样和分析实验之前，使用激光多普勒测速仪系统对流场进行了详细测量，以获得该流场中平均速度分布、相对湍流强度和τR分布的等值线图。本实验室开发的一种新的两点采样技术，可以从流场中的选定位置采集红细胞样本，从而建立局部剪切应力水平与细胞损伤之间的关系。发现导致初始溶血的τR阈值水平约为400牛顿每平方米（N/m2），低于该值时观察到零溶血的亚致死区域。
