LeBowitz J H, McMacken R
Adv Exp Med Biol. 1984;179:77-89. doi: 10.1007/978-1-4684-8730-5_8.
The bacteriophage lambda O and P protein replication initiators, in conjunction with six purified Escherichia coli replication proteins, replicate the single-stranded chromosomes of phages M13 and phi X174 to a duplex form. Several discrete steps are involved in this DNA synthesis reaction. In an ATP-dependent step that precedes priming, the lambda O and P proteins interact with the Escherichia coli dnaJ and dnaK proteins to transfer the bacterial dnaB protein onto DNA coated with single-stranded DNA binding protein. This creates a stable prepriming intermediate, isolable by gel filtration, that is rapidly primed and replicated upon the addition of primase and DNA polymerase III holoenzyme. Each of the eight proteins required for this nonspecific single strand replication reaction also have physiological roles in the replication of the bacteriophage lambda chromosome in vivo. We propose a scheme for the lambda O and P protein-dependent initiation of DNA synthesis that may be relevant to strand initiation events occurring during lambda DNA replication.
噬菌体λO和P蛋白复制起始因子，与六种纯化的大肠杆菌复制蛋白一起，将噬菌体M13和φX174的单链染色体复制成双链形式。该DNA合成反应涉及几个离散步骤。在引发之前的一个依赖ATP的步骤中，λO和P蛋白与大肠杆菌dnaJ和dnaK蛋白相互作用，将细菌dnaB蛋白转移到涂有单链DNA结合蛋白的DNA上。这产生了一种稳定的预引发中间体，可通过凝胶过滤分离，在加入引发酶和DNA聚合酶III全酶后迅速引发并复制。这种非特异性单链复制反应所需的八种蛋白质中的每一种在噬菌体λ染色体在体内的复制中也都具有生理作用。我们提出了一种与λO和P蛋白依赖性DNA合成起始相关的方案，该方案可能与λDNA复制过程中发生的链起始事件有关。
