Orlean P
Antonie Van Leeuwenhoek. 1984;50(4):341-8. doi: 10.1007/BF00394647.
A particulate glucosyltransferase prepared from budding and filamentous cultures of Candida albicans used uridine diphosphate glucose as sole glucosyl donor in a reaction (measured by following the incorporation of [14C]-glucose from UDP [14C]-glucose into polymer) stimulated by glucose-6-phosphate and inhibited by adenosine triphosphate and guanosine triphosphate. The radiolabelled reaction product was solubilized by alpha-amylase, and, on oxidation with periodate followed by reduction with borohydride and acid hydrolysis, yielded erythritol and glycerol in the ratio of 4 to 1. The radiolabelled glucosyl residues were attached to an endogenous acceptor of high molecular weight.
从白色念珠菌的芽殖和丝状培养物中制备的一种颗粒状葡糖基转移酶，在由6-磷酸葡萄糖刺激且被三磷酸腺苷和三磷酸鸟苷抑制的反应中（通过追踪[14C]-葡萄糖从尿苷二磷酸[14C]-葡萄糖掺入聚合物来测定），以尿苷二磷酸葡萄糖作为唯一的葡糖基供体。放射性标记的反应产物可被α-淀粉酶溶解，在用高碘酸盐氧化、随后用硼氢化钠还原并进行酸水解后，产生的赤藓醇和甘油的比例为4比1。放射性标记的葡糖基残基连接到一种高分子量的内源性受体上。
