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粗糙脉孢菌中的糖原合成。

Glycogen synthesis in the fungus Neurospora crassa.

作者信息

Fontana J D, Krisman C R

出版信息

Biochim Biophys Acta. 1978 May 3;540(2):183-9. doi: 10.1016/0304-4165(78)90130-7.

DOI:10.1016/0304-4165(78)90130-7
PMID:148915
Abstract

An enzymic activity, obtained from Neurospora crassa, catalyzing the incorporation of [14C]glucose from ADP-[14C]glucose into a glucan of the glycogen type, is described. The properties of the ADPglucose : glycogen glucosyltransferase as compared with those of the already known UDP glucose : glycogen glucosyltransferase were studied. The radioactive products obtained with UDP-14C]glucose or ADP-[14C]glucose released all the radioactivity as maltose after alpha or beta amylase treatment. Glucose 6-phosphate stimulated the synthetase when UDP-[14C]glucose was the substrate but the stimulation was much greater with ADP-[14C]glucose as glucosyl donor. Glucose 6-phosphate plus EGTA gave maximal stimulation. The system was completely dependent &on the presence of a 'primer' of the alpha 1 leads to 4 glucan type.

摘要

描述了一种从粗糙脉孢菌中获得的酶活性,该酶催化将ADP-[14C]葡萄糖中的[14C]葡萄糖掺入糖原类型的葡聚糖中。研究了ADP葡萄糖:糖原葡糖基转移酶与已知的UDP葡萄糖:糖原葡糖基转移酶相比的特性。用UDP-[14C]葡萄糖或ADP-[14C]葡萄糖获得的放射性产物在α或β淀粉酶处理后以麦芽糖形式释放出所有放射性。当UDP-[14C]葡萄糖作为底物时,6-磷酸葡萄糖刺激合成酶,但以ADP-[14C]葡萄糖作为葡糖基供体时刺激作用更大。6-磷酸葡萄糖加EGTA产生最大刺激。该系统完全依赖于α1→4葡聚糖类型的“引物”的存在。

相似文献

1
Glycogen synthesis in the fungus Neurospora crassa.粗糙脉孢菌中的糖原合成。
Biochim Biophys Acta. 1978 May 3;540(2):183-9. doi: 10.1016/0304-4165(78)90130-7.
2
Biosynthesis of glycogen in Neurospora crassa. Chemical composition and binding to glycogen of UDP-glucose: glycogen 4-alpha-glucosyltransferase.粗糙脉孢菌中糖原的生物合成。UDP-葡萄糖:糖原4-α-葡萄糖基转移酶的化学组成及与糖原的结合
J Biochem. 1979 Apr;85(4):907-14. doi: 10.1093/oxfordjournals.jbchem.a132422.
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Biosynthesis of glycogen in Neurospora crassa. Purification and properties of the UDPglucose:glycogen 4-alpha-glucosyltransferase.粗糙脉孢菌中糖原的生物合成。尿苷二磷酸葡萄糖:糖原4-α-葡糖基转移酶的纯化及性质
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Mode of action of glycogen branching enzyme from Neurospora crassa.粗糙脉孢菌糖原分支酶的作用模式。
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Stimulation by mammalian insulin of glycogen metabolism in a wall-less strain of Neurospora crassa.哺乳动物胰岛素对无细胞壁粗糙脉孢菌菌株糖原代谢的刺激作用。
Endocrinology. 1988 Feb;122(2):518-23. doi: 10.1210/endo-122-2-518.
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Biosynthesis of glycogen in Neurospora crassa. Kinetic mechanism of UDP-glucose: glycogen 4-alpha-glucosyltransferase.粗糙脉孢菌中糖原的生物合成。UDP-葡萄糖:糖原4-α-葡糖基转移酶的动力学机制。
J Biochem. 1978 Dec;84(6):1381-7. doi: 10.1093/oxfordjournals.jbchem.a132260.
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An assay for adenosine 5'-diphosphate (ADP)-glucose pyrophosphorylase that measures the synthesis of radioactive ADP-glucose with glycogen synthase.一种用于测定腺苷5'-二磷酸(ADP)-葡萄糖焦磷酸化酶的分析方法,该方法通过糖原合酶来测量放射性ADP-葡萄糖的合成。
Anal Biochem. 2004 Jan 1;324(1):52-9. doi: 10.1016/j.ab.2003.09.024.
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Effect of L-sorbose on polysaccharide synthetases of Neurospora crassa (glycogen- -1,3-glucan-morphology-cell wall-digitonin-particulate enzymes).L-山梨糖对粗糙脉孢菌多糖合成酶的影响(糖原-1,3-葡聚糖-形态-细胞壁-洋地黄皂苷-颗粒酶)
Proc Natl Acad Sci U S A. 1972 Feb;69(2):313-7. doi: 10.1073/pnas.69.2.313.
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Activation of membrane-bound adenylate cyclase by glucagon in Neurospora crassa.在粗糙脉孢菌中,胰高血糖素对膜结合腺苷酸环化酶的激活作用。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2870-3. doi: 10.1073/pnas.69.10.2870.
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Biosynthesis of glycogen in Neurospora crassa. Existence of a glucoproteic intermediate in the initiation process.粗糙脉孢菌中糖原的生物合成。起始过程中糖蛋白中间体的存在。
J Biochem. 1977 Jun;81(6):1587-94. doi: 10.1093/oxfordjournals.jbchem.a131617.

引用本文的文献

1
Glycogen biosynthesis via UDP-glucose in the ruminal bacterium Prevotella bryantii B1(4).通过尿苷二磷酸葡萄糖在瘤胃细菌普氏栖粪杆菌B1(4)中进行糖原生物合成。
Appl Environ Microbiol. 1997 Nov;63(11):4355-9. doi: 10.1128/aem.63.11.4355-4359.1997.
2
Role of nuclear glycogen synthase and cytoplasmic UDP glucose pyrophosphorylase in the biosynthesis of nuclear glycogen in HD33 Ehrlich-Lettré ascites tumor cells.核糖原合酶和细胞质尿苷二磷酸葡萄糖焦磷酸化酶在HD33 Ehrlich-Lettre腹水瘤细胞核糖原生物合成中的作用。
J Cell Biol. 1981 Jun;89(3):475-84. doi: 10.1083/jcb.89.3.475.