Charles M A, Sharma B, Dodson L E, Ownbey J, Noble S, Waldeck N, Brown G, True L, Nakane P, Suzuki M
Diabetes Res. 1984 Jul;1(2):95-103.
Since the mechanisms of rat islet allograft rejection are unknown, metabolic, histologic, and immunologic parameters were characterized during rejection. Syngeneic intraportal islet transplants in diabetic Lewis rats normalize glucose values without islet cellular infiltrates; after Wistar-Furth allografts, glucose values rapidly return to diabetic levels and infiltration of islets by mononuclear cells occurs. Using lymphocyte proliferative assays, allogeneic islets induce a 3-fold stimulation of lymphocytes derived from nondiabetic allograft recipients. Using cytotoxicity assays, cell-mediated cytotoxicity of allogeneic islet target cells although inefficient is 2-fold greater in diabetic allograft recipients than in syngeneic recipients. Antibody-dependent cellular cytotoxicity is not observed, whereas 14 days after allotransplantation, complement-dependent antibody-mediated cytotoxicity is elevated. These data suggest that islet allograft rejection using metabolic, histopathologic and immunologic methods is associated primarily with cell-mediated cytotoxicity related to histocompatibility antigens.
由于大鼠胰岛同种异体移植排斥反应的机制尚不清楚，因此在排斥反应过程中对代谢、组织学和免疫学参数进行了表征。糖尿病Lewis大鼠的同基因门静脉内胰岛移植可使血糖值正常化，且无胰岛细胞浸润；Wistar-Furth同种异体移植后，血糖值迅速恢复到糖尿病水平，单核细胞浸润胰岛。使用淋巴细胞增殖试验，同种异体胰岛可诱导非糖尿病同种异体移植受者来源的淋巴细胞产生3倍的刺激。使用细胞毒性试验，同种异体胰岛靶细胞的细胞介导细胞毒性虽然效率不高，但在糖尿病同种异体移植受者中比在同基因受者中高2倍。未观察到抗体依赖性细胞毒性，而在同种异体移植14天后，补体依赖性抗体介导的细胞毒性升高。这些数据表明，使用代谢、组织病理学和免疫学方法的胰岛同种异体移植排斥反应主要与与组织相容性抗原相关的细胞介导细胞毒性有关。
