Rae-Venter B, Reid L M
Cancer Res. 1980 Jan;40(1):95-100.
Thirty-two malignant human breast tumors were implanted s.c. in female nude mice. Seven tumors survived for two passages, and four were established into permanent transplantable tumor lines. The transplantable tumors have retained the histopathology of the original tumor throughout passaging in the nude mice. In addition, two of the transplantable tumors have low concentrations of estrogen receptor. Tissue culture of the original tumor specimens upon receipt resulted in epithelial outgrowth in 15 of 32 primary cultures. However, no permanent cell lines were established. Attempts to culture 23 tumors frozen with dimethyl sulfoxide upon receipt were unsuccessful. In contrast, establishment of cell strains was successful with tumor specimens cultured following passage in the nude mice; three cell strains were initiated from two of the transplantable tumors.
将32个人类恶性乳腺肿瘤皮下植入雌性裸鼠体内。7个肿瘤存活了两代，4个建立了永久性可移植肿瘤系。在裸鼠传代过程中，可移植肿瘤一直保留着原始肿瘤的组织病理学特征。此外，其中两个可移植肿瘤的雌激素受体浓度较低。收到原始肿瘤标本后进行组织培养，32个原代培养中有15个出现上皮生长。然而，没有建立永久性细胞系。收到时用二甲基亚砜冷冻保存的23个肿瘤进行培养未成功。相比之下，用在裸鼠体内传代后的肿瘤标本进行培养成功建立了细胞株；从两个可移植肿瘤中启动了三个细胞株。
