Hirohashi S, Shimosato Y, Kameya T, Nagai K, Tsunematsu R
Cancer Res. 1977 Sep;37(9):3184-9.
The human breast cancer (Br-10) serially transplanted to nude mice (BALB/c-nu/nu) grew well in female mice but very slowly or not at all in untreated male mice and female mice treated with 1 mg of testosterone i.m. twice a week. The growth in female mice was arrested by ovariectomy, and that in male mice was accelerated by 0.1 mg of estradiol i.m. once a week. Tumors in female and estrogenized male mice retained the original histology of duct carcinoma. Tumors in ovariectomized female, androgenized female, and male mice consisted of cells with smaller and more uniform nuclei, forming markedly dilated lumina in the first group and arranged in lobular patterns in the latter two groups. High-affinity 8 S and 4 S estrogen receptors were present in tumors transplanted to female nude mice, but no progesterone receptors were detected. These results provide experimental evidence for the hormone dependency of a human breast cancer in vivo and strongly suggest the important role of estrogen and androgen in the growth regulation of some estrogen receptor-positive human breast cancers.
将人乳腺癌（Br-10）连续移植到裸鼠（BALB/c-nu/nu）体内，该肿瘤在雌性小鼠体内生长良好，但在未经处理的雄性小鼠以及每周两次肌肉注射1毫克睾酮的雌性小鼠体内生长非常缓慢或根本不生长。雌性小鼠的肿瘤生长可通过卵巢切除术抑制，而雄性小鼠的肿瘤生长可通过每周一次肌肉注射0.1毫克雌二醇加速。雌性小鼠和雌激素化雄性小鼠体内的肿瘤保留了导管癌的原始组织学特征。去卵巢雌性小鼠、雄激素化雌性小鼠和雄性小鼠体内的肿瘤由细胞核更小且更均匀的细胞组成，在第一组中形成明显扩张的管腔，在后两组中呈小叶状排列。移植到雌性裸鼠体内的肿瘤中存在高亲和力的8S和4S雌激素受体，但未检测到孕激素受体。这些结果为人类乳腺癌在体内的激素依赖性提供了实验证据，并有力地表明雌激素和雄激素在某些雌激素受体阳性的人类乳腺癌生长调节中起重要作用。
