Naor Z, Catt K J
J Biol Chem. 1980 Jan 25;255(2):342-4.
Gonadotropin releasing hormone (GnRH) and its potent analog [D-Ser(tBu)6]des-Gly10-GnRH N-ethylamide elevate pituitary cyclic GMP levels while stimulating gonadotropin release in cultured pituitary cells. Addition of mycophenolic acid to pituitary cell cultures decreased basal and GnRH-induced cGMP production to undetectable levels, but did not reduce basal or GnRH-stimulated luteinizing hormone (LH) release. Elevation of endogenous cGMP levels by sodium nitroprusside, or addition of cGMP or its potent derivatives, was also without effect on basal or GnRH-stimulated LH release. These findings demonstrate that the elevation of intracellular cGMP during GnRH action does not mediate the release of LH by pituitary cells.
促性腺激素释放激素（GnRH）及其强效类似物[D-丝氨酸（叔丁基）6]去甘氨酸10-GnRH N-乙酰胺可提高垂体环鸟苷酸（cGMP）水平，同时刺激培养的垂体细胞释放促性腺激素。在垂体细胞培养物中添加霉酚酸可将基础和GnRH诱导的cGMP产生降低到无法检测的水平，但并未降低基础或GnRH刺激的促黄体生成素（LH）释放。硝普钠提高内源性cGMP水平，或添加cGMP或其强效衍生物，对基础或GnRH刺激的LH释放也没有影响。这些发现表明，GnRH作用期间细胞内cGMP的升高并不介导垂体细胞释放LH。
