在细胞松弛素B存在的情况下，感染SV40的小鼠细胞的DNA合成与多核化。

DNA synthesis and multinucleation of mouse cells infected with SV40 in the presence of cytochalasin B.

作者信息

Kurimura T, Hirano A

出版信息

J Gen Virol. 1980 Jan;46(1):237-42. doi: 10.1099/0022-1317-46-1-237.

Abstract

DNA synthesis and nuclear division of mouse cells, BALB/3T3, infected with SV40 were studied and were compared with those of SV40-transformed mouse cells, mKS-A TU-7. Both SV40-infected and SV40-transformed cells behaved similarly in the presence of cytochalasin B and differently from normal noninfected cells, BALB/3T3. The chemical inhibited cytokinesis of all the cell groups but the nuclear division was inhibited only in the case of non-infected BALB/3T3. With SV40-infected BALB/3T3, multinucleation occurred with the increase of input m.o.i. by SV40. SV40-infected BALB/3T3 could enter the second S phase after release from double thymidine block in the presence of cytochalasin B, while BALB/3T3 could not enter the second S phase. In bi- or multinucleated cells of SV40-infected BALB/3T3, asynchrony of DNA synthesis among nuclei in a cell was evident, as was the case with mKS-A TU-7.

摘要

对感染了SV40的小鼠细胞BALB/3T3的DNA合成和核分裂进行了研究，并与SV40转化的小鼠细胞mKS-A TU-7的DNA合成和核分裂进行了比较。在细胞松弛素B存在的情况下，感染SV40的细胞和SV40转化的细胞表现相似，与正常未感染的细胞BALB/3T3不同。这种化学物质抑制了所有细胞组的胞质分裂，但仅在未感染的BALB/3T3细胞中抑制了核分裂。对于感染SV40的BALB/3T3细胞，随着SV40感染复数的增加会出现多核化。在细胞松弛素B存在的情况下，感染SV40的BALB/3T3细胞在从双胸腺嘧啶阻断释放后可进入第二个S期，而BALB/3T3细胞则不能进入第二个S期。在感染SV40的BALB/3T3的双核或多核细胞中，细胞内各细胞核之间的DNA合成异步现象明显，mKS-A TU-7细胞也是如此。