Schafer A I, Alexander R W, Handin R I
Blood. 1980 Apr;55(4):649-54.
There is evidence that platelet activation in the coronary circulation may be important in the pathogenesis of myocardial ischemia. Since organic nitrate vasodilators are commonly used in coronary artery disease, we have studied the in vitro effects of these drugs on platelet function. Nitroglycerin, isosorbide dinitrate, and their biotransformation product, inorganic nitrite, inhibited platelet aggregation with collagen, epinephrine, arachidonate, and ionophore, and blocked both primary and secondary aggregation in response to ADP. Nitroglycerin was studied in more detail. Its inhibitory effect was reversible and not dependent on external calcium concentration. It inhibited arachidonic acid oxygenation as measured by the arachidonate-induced oxygen burst and malonaldehyde production. These effects were not due to an increase in intracellular cyclic AMP. This unusual generalized inhibition of platelet function by nitroglycerin possibly contributes to its beneficial effect in myocardial ischemia in part by attenuating platelet reactivity in the coronary circulation.
有证据表明，冠状动脉循环中的血小板活化在心肌缺血的发病机制中可能起重要作用。由于有机硝酸酯类血管扩张剂常用于冠状动脉疾病，我们研究了这些药物对血小板功能的体外作用。硝酸甘油、异山梨醇二硝酸酯及其生物转化产物无机亚硝酸盐可抑制血小板与胶原、肾上腺素、花生四烯酸和离子载体的聚集，并阻断对ADP的初级和次级聚集。对硝酸甘油进行了更详细的研究。其抑制作用是可逆的，且不依赖于细胞外钙浓度。它抑制了由花生四烯酸诱导的氧爆发和丙二醛生成所测定的花生四烯酸氧化。这些作用并非由于细胞内环磷酸腺苷增加所致。硝酸甘油对血小板功能这种不寻常的广泛抑制作用，可能部分地通过减弱冠状动脉循环中的血小板反应性，对其在心肌缺血中的有益作用有所贡献。
