Nash J R, Everson N W, Wood R F, Bell P R
Transplantation. 1980 Mar;29(3):206-8. doi: 10.1097/00007890-198003000-00008.
Pancreatic islet cell allotransplantation has considerable potential in the treatment of diabetes mellitus. However, isolated islets appear to be particularly sensitive to rejection and attempts to prolong their survival in experimental animals have so far met with only limited success. In this study the effect of two macrophage suppressing agents, silica and carrageenan, has been investigated in a rat model. Islets from (August x AS) F1 donors were transplanted into AS recipients. Of 13 animals treated with i.v. carrageenan (5 mg/kg), only one had prolongation of graft survival compared to controls. There was indefinite survival in 7 of the 16 animals treated with i.p. silica (50 mg/100 g) and 2 additional animals died normoglycaemic. In an attempt to measure the effectiveness of these two agents in suppressing macrophage activity, the ability of the "fixed" macrophages of the mononuclear phagocytic system to clear particles from the circulation was measured using 125I-labelled microaggregated albumin. Although both carrageenan and silica reduced clearance values, it proved impossible to correlate the degree and duration of this suppression with the success or otherwise of the islet cell allografts.
胰岛细胞同种异体移植在糖尿病治疗方面具有巨大潜力。然而，分离出的胰岛似乎对排斥反应特别敏感，迄今为止，在实验动物中延长其存活时间的尝试仅取得了有限的成功。在本研究中，在大鼠模型中研究了两种巨噬细胞抑制因子——二氧化硅和角叉菜胶的作用。将（奥古斯塔×AS）F1供体的胰岛移植到AS受体中。静脉注射角叉菜胶（5毫克/千克）治疗的13只动物中，与对照组相比，只有1只的移植物存活时间延长。腹腔注射二氧化硅（50毫克/100克）治疗的16只动物中有7只移植物存活时间不确定，另外2只动物血糖正常时死亡。为了测量这两种因子抑制巨噬细胞活性的效果，使用125I标记的微聚白蛋白测量单核吞噬系统中“固定”巨噬细胞从循环中清除颗粒的能力。虽然角叉菜胶和二氧化硅都降低了清除值，但事实证明无法将这种抑制的程度和持续时间与胰岛细胞同种异体移植的成功与否联系起来。
