Rogers P A, Brenton D P, Hopkinson D A
Ann Hum Genet. 1980 Jan;43(3):213-26. doi: 10.1111/j.1469-1809.1980.tb01555.x.
A study has been made of the effects of phytohaemagglutinin on the gene expression of the glycolytic enzymes in cultured human lymphocytes. All the enzymes were found to show an average increase in activity of between 160% and 360% in stimulated cells, but the increases were greater for the enzymes comprising the second half of the pathway. The enzyme activities in stimulated cells, cultured for 72 h, were similar to the activities measured in long-term lymphoid lines. Starch-gel electrophoresis was used to examine the isozyme patterns of the enzymes before and after exposure of the lymphocytes to PHA. Six of the enzymes showed isozyme patterns unchanged by stimulation. Four of the enzymes, aldolase, triosephosphate isomerase, enolase and lactate dehydrogenase, showed different isozyme patterns in stimulated cells from those seen in uncultured or unstimulated cells. The electrophoretic results showed a good correlation in isozyme pattern between uncultured lymphocytes and cultured unstimulated lymphocytes, and between PHA-stimulated lymphocytes and long-term lymphoid lines.
一项关于植物血凝素对培养的人淋巴细胞中糖酵解酶基因表达影响的研究已经展开。结果发现，在受刺激的细胞中，所有这些酶的活性平均增加了160%至360%，但对于构成该途径后半部分的酶，其增加幅度更大。培养72小时的受刺激细胞中的酶活性与长期淋巴细胞系中测得的活性相似。淀粉凝胶电泳用于检测淋巴细胞暴露于植物血凝素前后这些酶的同工酶谱。六种酶的同工酶谱在刺激后未发生变化。四种酶，即醛缩酶、磷酸丙糖异构酶、烯醇化酶和乳酸脱氢酶，在受刺激细胞中的同工酶谱与未培养或未受刺激细胞中的不同。电泳结果表明，未培养的淋巴细胞与培养的未受刺激淋巴细胞之间，以及植物血凝素刺激的淋巴细胞与长期淋巴细胞系之间，同工酶谱具有良好的相关性。
