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公牛和公羊精子尾部及线粒体中的脱氧核糖核酸酶II

DNase II in bull and ram sperm tail and mitochondria.

作者信息

Fisher J, Bartoov B

出版信息

Arch Androl. 1980 Mar;4(2):157-70. doi: 10.3109/01485018008986483.

DOI:10.3109/01485018008986483
PMID:6244797
Abstract

Extracts of bull and ram sperm tails were prepared by DTT and CTAB treatment. Such extracts contained deoxyribonuclease II, degrading only native double-stranded DNA at acid pH (3.9--4.5). At least three distinguishable DNase II activities were found in these extracts as judged by their sensitivity to thiol compounds and various anions and cations. The deoxyribonuclease II activity is apparently located in or in association with the mitochondria.

摘要

通过二硫苏糖醇(DTT)和十六烷基三甲基溴化铵(CTAB)处理制备公牛和公羊精子尾部提取物。此类提取物含有脱氧核糖核酸酶II,其仅在酸性pH值(3.9 - 4.5)下降解天然双链DNA。根据它们对硫醇化合物以及各种阴离子和阳离子的敏感性判断,在这些提取物中发现了至少三种可区分的脱氧核糖核酸酶II活性。脱氧核糖核酸酶II活性显然位于线粒体中或与线粒体相关联。

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