Hamlin J L, Pardee A B
In Vitro. 1978 Jan;14(1):119-27. doi: 10.1007/BF02618178.
Eukaryotic DNA is functionally divided into thousands of replicons, each of which may be duplicated at a characteristic time within the DNA synthetic (S) period. Our approach toward an understanding of the molecular mechanisms which control orderly eukaryotic DNA synthesis has been: (a) to devise a method of cell synchrony in a suitable tissue culture system wherein all cells in the population enter and traverse the S period with a high degree of synchrony; (b) to determine, utilizing this system, precisely when during the S period critical events and macromolecular syntheses occur; and (c) to examine, by polyacrylamide-gel electrophoresis, the spectrum of proteins which become associated with chromatin during the S period in such a way as to suggest their involvement with DNA synthesis. Possible mechanisms for control are discussed based on the results presented here.
真核生物的DNA在功能上被分为数千个复制子，每个复制子可能在DNA合成（S）期内的特定时间进行复制。我们为理解控制真核生物DNA有序合成的分子机制所采用的方法是：（a）在合适的组织培养系统中设计一种细胞同步化方法，使群体中的所有细胞都能高度同步地进入并穿过S期；（b）利用该系统精确确定在S期的何时发生关键事件和大分子合成；（c）通过聚丙烯酰胺凝胶电泳检查在S期与染色质结合的蛋白质谱，以此表明它们与DNA合成有关。基于此处给出的结果讨论了可能的控制机制。
