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一种用于纯化具有序列特异性的DNA结合蛋白的新方法。

A new method for the purification of DNA-binding proteins with sequence specificity.

作者信息

Weideli H, Gehring W J

出版信息

Eur J Biochem. 1980 Feb;104(1):5-11. doi: 10.1111/j.1432-1033.1980.tb04392.x.

DOI:10.1111/j.1432-1033.1980.tb04392.x
PMID:6245871
Abstract

We describe a technique for a rapid and efficient isolation and purification of proteins binding to defined DNA sequences. Cloned double-stranded DNA was covalently coupled to m-aminobenzyloximethylcellulose in order to purify proteins which recognize and bind to specific sequences on the DNA. The purification of two DNA-binding proteins from Drosophila melanogaster is demonstrated using the respective cloned DNA sequences.

摘要

我们描述了一种快速高效地分离和纯化与特定DNA序列结合的蛋白质的技术。将克隆的双链DNA与间氨基苄基氧甲基纤维素共价偶联，以纯化识别并结合DNA上特定序列的蛋白质。利用各自克隆的DNA序列，展示了从黑腹果蝇中纯化两种DNA结合蛋白的过程。

相似文献

1
A new method for the purification of DNA-binding proteins with sequence specificity.一种用于纯化具有序列特异性的DNA结合蛋白的新方法。
Eur J Biochem. 1980 Feb;104(1):5-11. doi: 10.1111/j.1432-1033.1980.tb04392.x.
2
Purification of a protein from unfertilized eggs of Drosophila with specific affinity for a defined DNA sequence and the cloning of this DNA sequence in bacterial plasmids.从果蝇未受精卵中纯化出对特定DNA序列具有特异性亲和力的蛋白质，并将该DNA序列克隆到细菌质粒中。
Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:693-700. doi: 10.1101/sqb.1978.042.01.071.
3
Purification of two proteins with specific affinity for defined DNA sequences from Drosophila and cloning of these sequences in bacterial plasmids [proceedings].从果蝇中纯化出对特定DNA序列具有特异性亲和力的两种蛋白质，并将这些序列克隆到细菌质粒中[会议论文集]
Ann Microbiol (Paris). 1978 Nov-Dec;129 B(4):513-4.
4
Isolation and characterisation of dhel II, a DNA helicase from Drosophila melanogaster embryos stimulated by Escherichia coli-type single-stranded-DNA-binding proteins.从黑腹果蝇胚胎中分离并鉴定Dhel II，一种受大肠杆菌型单链DNA结合蛋白刺激的DNA解旋酶。
Eur J Biochem. 1996 Jan 15;235(1-2):152-8. doi: 10.1111/j.1432-1033.1996.00152.x.
5
Purification and characterisation of a DNA helicase, dheI I, from Drosophila melanogaster embryos.从黑腹果蝇胚胎中纯化和鉴定一种DNA解旋酶dheI I
Nucleic Acids Res. 1995 Nov 11;23(21):4443-50. doi: 10.1093/nar/23.21.4443.
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Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.果蝇中选择性结合左旋Z-DNA的蛋白质的分离。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7729-33. doi: 10.1073/pnas.79.24.7729.
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Immunoglobulin switch region-like sequences in Drosophila melanogaster.黑腹果蝇中的免疫球蛋白转换区样序列。
Nucleic Acids Res. 1982 Jul 24;10(14):4203-14. doi: 10.1093/nar/10.14.4203.
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Recombinant yeast mtDNA helicases. Purification and functional assays.
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Cloning of the replication origin from Drosophila virilis mitochondrial DNA.果蝇线粒体DNA复制起点的克隆
Biochem Biophys Res Commun. 1979 Dec 28;91(4):1321-9. doi: 10.1016/0006-291x(79)91211-7.
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Translation of Drosophila melanogaster sequences in Escherichia coli.黑腹果蝇序列在大肠杆菌中的翻译。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):5041-5. doi: 10.1073/pnas.74.11.5041.

引用本文的文献

1
Characterization of Drosophila DNA-binding protein DB-2: demonstration of its sequence-specific interaction with DNA.果蝇DNA结合蛋白DB-2的特性：证明其与DNA的序列特异性相互作用。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3773-7. doi: 10.1073/pnas.77.7.3773.
2
Preferential binding of estrogen-receptor complex to a region containing the estrogen-dependent hypomethylation site preceding the chicken vitellogenin II gene.雌激素受体复合物与鸡卵黄生成素II基因前含雌激素依赖性低甲基化位点区域的优先结合。
Proc Natl Acad Sci U S A. 1984 Jan;81(2):429-33. doi: 10.1073/pnas.81.2.429.
3
Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
使用蛋白质印迹法和特异性DNA探针来鉴定识别转铁蛋白受体基因启动子区域的核蛋白。
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741-4. doi: 10.1073/pnas.82.20.6741.