Lehoux J G, Preiss B
Endocrinology. 1980 Jul;107(1):215-23. doi: 10.1210/endo-107-1-215.
In the adrenal gland of the hamster, the activity of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase [mevalonate:NADP+ oxidoreductase (acylating CoA); EC 1.1.1.34] is mainly located in the postmitochondria fraction. This enzyme exhibits a diurnal rhythm, with a maximum at 1900 h. Peak activity levels are about 1667 pmol/mg protein . min, roughly 8 times the minimum levels. Plasma corticosteroid concentrations closely parallel reductase activity levels. The administration of ACTH or metyrapone (a drug which increases in vivo ACTH secretion) to hamsters enhanced the adrenal reductase activity, whereas aminoglutethimide, an inhibitor of the side chain cleavage of cholesterol, diminished it. The feeding of a 5% cholesterol diet and treatment with 4-aminopyrazolopyrimidine resulted in an increase and a decrease in plasma cholesterol, respectively. Neither treatment significantly altered adrenal 3-hydroxy-3-methylglutaryl coenzyme A reductase activity or the cholesterol content of the gland. Hamster adrenals contain very little free cholesterol (7-11 microgram/mg protein) and even less esterified cholesterol (0.5-2.0 microgram/mg protein). These findings suggest that in the hamster the adrenal gland may be largely autonomous in cholesterol production.
在仓鼠的肾上腺中，3-羟基-3-甲基戊二酰辅酶A还原酶[mevalonate:NADP+氧化还原酶（酰化辅酶A）；EC 1.1.1.34]的活性主要位于线粒体后部分。这种酶呈现昼夜节律，在1900时达到最大值。峰值活性水平约为1667 pmol/mg蛋白质·分钟，约为最低水平的8倍。血浆皮质类固醇浓度与还原酶活性水平密切平行。给仓鼠注射促肾上腺皮质激素（ACTH）或美替拉酮（一种可增加体内ACTH分泌的药物）可增强肾上腺还原酶活性，而胆固醇侧链裂解抑制剂氨鲁米特则会降低其活性。喂食5%胆固醇饮食和用4-氨基吡唑并嘧啶治疗分别导致血浆胆固醇升高和降低。两种治疗均未显著改变肾上腺3-羟基-3-甲基戊二酰辅酶A还原酶活性或腺体的胆固醇含量。仓鼠肾上腺中游离胆固醇含量极少（7 - 11微克/毫克蛋白质），酯化胆固醇含量甚至更低（0.5 - 2.0微克/毫克蛋白质）。这些发现表明，在仓鼠中，肾上腺在胆固醇生成方面可能很大程度上是自主的。
