从正常人尿液中分离出的一种新型致高血压化合物的体外类固醇生成特性。

In vitro steroidogenic properties of a new hypertension-producing compound isolated from normal human urine.

作者信息

Bravo E L, Saito I, Zanella T, Sen S, Bumpus F M

出版信息

J Clin Endocrinol Metab. 1980 Jul;51(1):176-8. doi: 10.1210/jcem-51-1-176.

Abstract

The steroidogenic properties of a glycoprotein fraction (ASF), isolated from normal human urine, were studied in cat adrenal capsular collagenase-dispersed cells and its effects compared to those of ACTH and Angiotensin II (AII). ACTH, AII and ASF induced dose-related increases in both aldosterone and cortisol production. In order of potency, ACTH = AII greater than ASF in stimulating aldosterone production and ACTH greater than AII greater than ASF in stimulating cortisol production. Increases in cAMP accompanied the steroidogenic response to ACTH but not to AII or ASF. The response to AII, but not to ASF, was inhibited (87% of normal) by equimolar concentrations of [Sar1, Thr8]AII, a specific AII antagonist. These results suggest that ASF is a true aldosterone secretagogue and that it initiates steroidogenesis by mechanisms similar to those of AII. However, the inability to block it effect with a specific antagonist of AII provides evidence for its action on a separate receptor site.

摘要

从正常人尿液中分离出的一种糖蛋白组分（ASF）的类固醇生成特性，在猫肾上腺皮质包膜胶原酶分散细胞中进行了研究，并将其作用与促肾上腺皮质激素（ACTH）和血管紧张素II（AII）的作用进行了比较。ACTH、AII和ASF均可诱导醛固酮和皮质醇生成呈剂量相关增加。就效力而言，在刺激醛固酮生成方面，ACTH = AII＞ASF；在刺激皮质醇生成方面，ACTH＞AII＞ASF。cAMP的增加伴随着对ACTH的类固醇生成反应，但不伴随对AII或ASF的反应。等摩尔浓度的特异性AII拮抗剂[Sar1, Thr8]AII可抑制对AII的反应（降至正常的87%），但不抑制对ASF的反应。这些结果表明，ASF是一种真正的醛固酮促分泌素，并且它通过与AII类似的机制启动类固醇生成。然而，无法用AII的特异性拮抗剂阻断其作用，这为其作用于一个独立的受体位点提供了证据。