Barber R, Ray K P, Butcher R W
J Cyclic Nucleotide Res. 1980;6(1):15-24.
The kinetic parameters that determine the accumulation of cAMP in WI-38 cells stimulated with prostaglandin E1 have been determined at 37 degrees C and at lower temperatures. For desensitized cells, a reduction of temperatures from 37 degrees to 25 degrees C reduced both rate of synthesis and rate of elimination of cAMP by about 40%. The steady-state accumulation was, therefore, about the same at both temperatures. The extent of desensitization was also shown to be comparable at the two temperatures. It can be inferred that there was appreciable desensitization at 4 degrees C after a period of stimulation of less than one hour. This is contrasted with the behavior of C6-2B glioma cells at the same temperature. Escape of cAMP through the plasma membrane showed a greater temperature dependence than any of the other processes concerned with cAMP accumulation.
已在37℃和更低温度下测定了决定用前列腺素E1刺激的WI-38细胞中cAMP积累的动力学参数。对于脱敏细胞，温度从37℃降至25℃时，cAMP的合成速率和消除速率均降低约40%。因此，在这两个温度下的稳态积累大致相同。在这两个温度下脱敏的程度也相当。可以推断，在刺激不到一小时后，4℃时存在明显的脱敏现象。这与相同温度下C6-2B胶质瘤细胞的行为形成对比。cAMP通过质膜的逸出比与cAMP积累相关的任何其他过程表现出更大的温度依赖性。
