病毒作为研究哺乳动物细胞分子生物学的工具。

Viruses as tools for studies on the molecular biology of mammalian cells.

Doerfler W, Stabel S, Ibelgaufts H, Sutter D, Neumann R, Deuring R, Scheidtmann K H, Winterhoff U

Arzneimittelforschung. 1980;30(3a):558-69.

Abstract

Multiple copies of intact adenovirus type 12 (Ad12) DNA are integrated into the DNA of Ad12-transformed hamster and Ad12-induced rat brain tumor cells. Free viral DNA is not present in the Ad12-transformed lines investigated. 2. Only few sites of integration are found in Al2-transformed hamster and Ad12-induced rat brain tumor cells. Integration may have occurred into repetitive cellular sequences at selective sites. These sites may be different in different cell lines, however, none of these sites has been analyzed in sufficient detail. 3. Three lines of Ad12-induced rat brain tumor cells exhibit identical patterns of integration. These lines have been derived from three brain tumors in one animal and may have evolved from the same transformed cell. 4. In Ad12-induced rat brain tumor cells early and late segments of the viral genome are expressed as polysome-associated messenger RNA. 5. In human cells productively infected with adenovirus type 2 (Ad2), a large number of viral genome copies are linked to cellular DNA early postinfection. There are only a few sites of recombination (illegitimate?) which probably lie in repetitive cellular DNA sequences. The functional significance of this frequent recombination is unknown.

摘要

完整的12型腺病毒（Ad12）DNA的多个拷贝整合到Ad12转化的仓鼠细胞和Ad12诱导的大鼠脑肿瘤细胞的DNA中。在所研究的Ad12转化细胞系中不存在游离的病毒DNA。2. 在Ad12转化的仓鼠细胞和Ad12诱导的大鼠脑肿瘤细胞中仅发现少数整合位点。整合可能发生在选择性位点的重复细胞序列中。然而，这些位点在不同细胞系中可能不同，并且尚未对这些位点中的任何一个进行足够详细的分析。3. 三株Ad12诱导的大鼠脑肿瘤细胞系表现出相同的整合模式。这些细胞系源自一只动物的三个脑肿瘤，可能由同一个转化细胞进化而来。4. 在Ad12诱导的大鼠脑肿瘤细胞中，病毒基因组的早期和晚期片段表达为多核糖体相关的信使RNA。5. 在被2型腺病毒（Ad2）有效感染的人细胞中，大量病毒基因组拷贝在感染后早期与细胞DNA相连。只有少数重组位点（可能是非法的？），它们可能位于重复的细胞DNA序列中。这种频繁重组的功能意义尚不清楚。