Hanafusa T, Wang L H, Anderson S M, Karess R E, Hayward W S, Hanafusa H
Proc Natl Acad Sci U S A. 1980 May;77(5):3009-13. doi: 10.1073/pnas.77.5.3009.
The src gene present in all avian sarcoma viruses is not present in the genome of Fujinami sarcoma virus, a potent sarcoma-inducing virus in chickens. Fujinami virus is defective and requires helper virus for replication. RNA from a mixture of helper and transforming viruses consists of two components, 35S and 28S. Oligonucleotide fingerprinting of each RNA component revealed that the 35S component was identical to the RNA of the helper virus. Thus, the genome of Fujinami virus must be the 28S RNA, which corresponds approximately to a molecular weight of 1.7 x 10(6) or 5300 nucleotides. Fujinami viral RNA shares several oligonucleotides with helper viral RNA at both 3' and 5' ends but contains a unique sequence of at least 3000 nucleotides in the middle of the genome. Fujinami viral RNA contains no src-specific oligonucleotides of the Rous sarcoma virus genome and did not hybridize with DNA complementary to the src sequences. The 60,000-dalton src protein of Rous sarcoma virus was undetectable in Fujinami virus-transformed cells. Instead, these transformed cells contain a protein of 140,000 daltons precipitable by antisera against virion proteins, which is likely to be the transforming protein of this virus.
所有禽肉瘤病毒中存在的src基因在鸡肉瘤诱导能力很强的藤浪肉瘤病毒基因组中并不存在。藤浪病毒有缺陷，需要辅助病毒才能复制。辅助病毒和转化病毒混合物中的RNA由35S和28S两个组分组成。对每个RNA组分进行寡核苷酸指纹分析表明，35S组分与辅助病毒的RNA相同。因此，藤浪病毒的基因组必定是28S RNA，其分子量约为1.7×10⁶或5300个核苷酸。藤浪病毒RNA在3'和5'末端与辅助病毒RNA共有几个寡核苷酸，但在基因组中间含有至少3000个核苷酸的独特序列。藤浪病毒RNA不包含劳氏肉瘤病毒基因组的src特异性寡核苷酸，也不与src序列互补的DNA杂交。在藤浪病毒转化的细胞中检测不到劳氏肉瘤病毒60000道尔顿的src蛋白。相反，这些转化细胞含有一种140000道尔顿的蛋白，可被抗病毒粒子蛋白的抗血清沉淀，这可能是该病毒的转化蛋白。
Zotero 插件
