Wang Yixin, Li Jianliang, Li Yang, Fang Lichun, Sun Xiaolong, Chang Shuang, Zhao Peng, Cui Zhizhong
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Tai'an, 271018, Shandong, China.
Virus Genes. 2016 Jun;52(3):365-71. doi: 10.1007/s11262-016-1301-6. Epub 2016 Apr 25.
Transduction of oncogenes by ALVs and generation of acute transforming viruses is common in natural viral infections. In order to understand the molecular basis for the rapid oncogenicity of Fu-J, an acutely transforming avian leukosis virus isolated from fibrosarcomas in crossbreed broilers infected with subgroup J avian leukosis virus (ALV-J) in China, complete genomic structure of Fu-J virus was determined by PCR amplification and compared with those of Fu-J1, Fu-J2, Fu-J3, Fu-J4, and Fu-J5 reported previously. The results showed that the genome of Fu-J was defective, with parts of gag gene replaced by the complete v-fps oncogene and encoded a 137 kDa Gag-fps fusion protein. Sequence analysis revealed that Fu-J and Fu-J1 to Fu-J5 were related quasi-species variants carrying different lengths of v-fps oncogenes generated from recombination between helper virus and c-fps gene. Comparison of virus carrying v-fps oncogene also gave us a glimpse of the molecular characterization and evolution process of the acutely transforming ALV.
禽白血病病毒（ALV）转导致癌基因并产生急性转化病毒在自然病毒感染中很常见。为了了解中国从感染J亚群禽白血病病毒（ALV-J）的杂交肉鸡纤维肉瘤中分离出的急性转化型禽白血病病毒Fu-J快速致癌性的分子基础，通过PCR扩增确定了Fu-J病毒的完整基因组结构，并与先前报道的Fu-J1、Fu-J2、Fu-J3、Fu-J4和Fu-J5的基因组结构进行了比较。结果表明，Fu-J的基因组存在缺陷，其gag基因的部分被完整的v-fps致癌基因取代，并编码一种137 kDa的Gag-fps融合蛋白。序列分析表明，Fu-J与Fu-J1至Fu-J5是相关的准种变体，它们携带由辅助病毒与c-fps基因重组产生的不同长度的v-fps致癌基因。对携带v-fps致癌基因的病毒进行比较，也让我们初步了解了急性转化型ALV的分子特征和进化过程。
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