裂褶菌核DNA的序列组织

Sequence organization of the nuclear DNA of Schizophyllum commune.

作者信息

Dons J J, Wessels J G

出版信息

Biochim Biophys Acta. 1980 May 30;607(3):385-96. doi: 10.1016/0005-2787(80)90149-5.

Abstract

Several methods were used to characterize the organization of repetitive DNA in the fungus Schizophyllum commune. They all failed to show interspersion of repetitive sequences among single copy sequences. Saturation hybridization showed that 2.2% of the double-stranded nuclear DNA coded for rRNA. The size of the ribosomal cistron (11.9.10(6) daltons) was determined by restriction enzyme analysis. From these values it was calculated that about 6% of the nuclear DNA consisted of ribosomal cistrons, which approx. equals the amount of repetitive DNA present. Thus, this simple sequence organization in Schizophyllum commune is fundamentally different from organization patterns in higher eukaryotes.

摘要

使用了几种方法来表征裂褶菌中重复DNA的组织形式。所有这些方法都未能显示出重复序列在单拷贝序列中的散布情况。饱和杂交表明，双链核DNA的2.2%编码rRNA。通过限制酶分析确定了核糖体顺反子的大小（11.9×10⁶道尔顿）。根据这些数值计算得出，约6%的核DNA由核糖体顺反子组成，这大约等于存在的重复DNA的量。因此，裂褶菌中的这种简单序列组织形式与高等真核生物中的组织模式根本不同。