Claesson H E
Biochim Biophys Acta. 1980 Jun 23;618(3):399-406. doi: 10.1016/0005-2760(80)90258-1.
Arachidonic acid and prostaglandin H2 elevate the levels of adenosine 3':5'-monophosphate (cyclic AMP) in Balb/c 3T3 fibroblasts. This effect was inhibited by 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid, an inhibitor of prostaglandin I2 synthase (Claesson, H.-E., Lindgren, J.A. and Hammarstr!om, S. (1977) FEBS Lett. 81, 415-418). After addition of arachidonic acid to 3T3 cultures, cellular cyclic AMP levels and growth medium concentrations of 6-ketoprostaglandin F1 alpha (degradation product of prostaglandin I2) were quantitatively determined. The stimulatory effect of exogenously-added prostaglandin I2 on cellular cyclic AMP levels was also determined. The results indicate that the endogenous production of prostaglandin I2 is sufficient to explain the stimulatory action of arachidonic acid on cyclic AMP formation in 3T3 fibroblasts.
花生四烯酸和前列腺素H2可提高Balb/c 3T3成纤维细胞中3':5'-单磷酸腺苷（环磷酸腺苷）的水平。这种效应被15-氢过氧-5,8,11,13-二十碳四烯酸抑制，15-氢过氧-5,8,11,13-二十碳四烯酸是前列腺素I2合酶的一种抑制剂（克莱松，H.-E.，林德格伦，J.A.和哈马斯特伦，S.（1977年）《欧洲生物化学学会联合会快报》81，415 - 418）。在向3T3培养物中添加花生四烯酸后，对细胞环磷酸腺苷水平以及6-酮前列腺素F1α（前列腺素I2的降解产物）的生长培养基浓度进行了定量测定。还测定了外源性添加的前列腺素I2对细胞环磷酸腺苷水平的刺激作用。结果表明，前列腺素I2的内源性产生足以解释花生四烯酸对3T3成纤维细胞中环磷酸腺苷形成的刺激作用。
