用纯化的肾钠钾ATP酶重构的磷脂囊泡的电子显微镜观察。
Electron microscopy of phospholipid vesicles reconstituted with purified renal Na,K-ATPase.
作者信息
Skriver E, Maunsbach A B, Anner B M, Jørgensen P L
出版信息
Cell Biol Int Rep. 1980 Jun;4(6):585-91. doi: 10.1016/0309-1651(80)90025-9.
PMID:6249502
Abstract
Purified Na,K-ATPase after reconstitution into phospholipid vesicles catalyzed an active coupled transport with a ratio close to 3Na/2K. A uniform population of closed vesicles with average diameters close to 900 A are observed after freeze-fracture and thin sectioning. After freeze-fracture intramembranous particles with diameters of 80-100 A are observed. The data suggest that these particles correspond to Na,K-ATPase molecules.
摘要
纯化后的 Na,K-ATP 酶重构到磷脂囊泡中后，催化了一种活性偶联转运，其比例接近 3 个钠离子/2 个钾离子。冷冻断裂和超薄切片后观察到平均直径接近 900 埃的均匀封闭囊泡群体。冷冻断裂后观察到直径为 80 - 100 埃的膜内颗粒。数据表明这些颗粒对应于 Na,K-ATP 酶分子。
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