铜与其他微量元素组合对胰蛋白酶活性的影响（作者译）
[Influence of copper in combination with other trace elements on the activity of trypsin (author's transl)].
作者信息
Kirchgessner M, Steinhart H, Wieninger-Rustemeyer R
出版信息
Int J Vitam Nutr Res. 1980;50(2):179-84.
The activity of trypsin was determined by using N alpha-Benzoyl-L-arginin-p-nitroanilid (L-BAPA) as a substrate, and adding either the elements Cu, Zn, Fe, Ni, and Co alone or in combination with copper in the concentration range of 0.9 x 10(-7) to 0.9 x 10(-4) moles Men+/l. Cu and Zn don't elevate the activity of trypsin but if they are added separately in very small concentrations. They lower the activity at higher concentrations. The other trace elements elevate the trypsin activity over the whole concentration range investigated. When copper is used in combination with the other trace elements there is no additional effect on trypsin activity. Here activities are somewhere in between the values when elements are added separately. A competing effect for the bounding sites of the trypsin is supposed. In this way it is possible to explain the non-linearity of the turnover rate curves of the experiments with the combined trace elements.
以Nα-苯甲酰-L-精氨酸对硝基苯胺（L-BAPA）为底物，测定胰蛋白酶的活性，并添加单独的元素铜、锌、铁、镍和钴，或与铜组合添加，浓度范围为0.9×10⁻⁷至0.9×10⁻⁴摩尔/升金属离子。铜和锌不会提高胰蛋白酶的活性，但如果以非常低的浓度单独添加，它们在较高浓度时会降低活性。在整个研究的浓度范围内，其他微量元素会提高胰蛋白酶的活性。当铜与其他微量元素组合使用时，对胰蛋白酶活性没有额外影响。此处的活性介于单独添加元素时的值之间。推测存在对胰蛋白酶结合位点的竞争效应。通过这种方式，可以解释组合微量元素实验中周转率曲线的非线性。
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