Blasdel G G, Pettigrew J D
J Physiol. 1978 Jan;274:601-19. doi: 10.1113/jphysiol.1978.sp012169.
The reversibility of monocular deprivation was tested physiologically in twelve kittens which had received varying amounts of normal visual experience prior to unilateral eye closure. Recordings obtained from cells in cortical area 17 indicated that the effects of monocular deprivation are reversible in cats as old as 8 weeks if the animals have been allowed normal visual experience before eye closure.1. Four kittens had their right eyes closed at 3, 4, and 5 weeks of age. All animals were reverse sutured at about 8 weeks of age, and after 2 weeks forced usage of the deprived eye, each animal was tested physiologically for recovery. Single unit recordings revealed a consistent relation between the extent to which the effects of initial deprivation could reverse and the age of the animal at the time of this deprivation. Recordings from a fifth, 8 week old kitten, which had been monocularly deprived for 3 weeks, ensured that our shortest period of deprivation induces a severe deficit.2. Two kittens which were reared in the dark for 4 and 5 weeks before unilateral eye closure, failed to show substantial (greater than 20%) reversal when reverse lid-sutured at 8 weeks of age. This failure suggests that kittens having their eyes closed after normal experience show enhanced reversibility, at least in part, on account of their early experience and not necessarily because of shorter deprivation. A litter-mate of one of these kittens was allowed 9 days of normal vision after dark rearing and before lid suture. Recordings which were performed both before and after 6 days of reverse suture demonstrated a near total (85%) reversal in ocular dominance.3. Three animals, monocularly deprived after some normal experience, recovered substantial numbers of binocular cells after both eyes had been allowed to remain open. In one of these cats the effectiveness of monocular deprivation was confirmed physiologically before eye opening. These findings contrast with those of other workers who have failed to find many binocular cells in kittens that had been allowed to recover, with eye opening, from monocular deprivation which had lasted since birth.4. One kitten was reared for 2 weeks (3-5 weeks of age) with alternating monocular deprivation. Single unit recordings from this cat, which were made after four additional weeks of unilateral eye closure, revealed some cells which had resisted monocular deprivation and which responded strongly to stimulation of the deprived eye. Reverse suture in the same animal led to the recovery of many cells (36%), dominated by the initially deprived eye, but did not result in a reversal in ocular dominance.
在12只小猫身上对单眼剥夺的可逆性进行了生理学测试，这些小猫在单侧眼睑闭合前有过不同程度的正常视觉体验。从17区皮质细胞获得的记录表明，如果动物在眼睑闭合前有过正常视觉体验，那么在8周龄的猫身上，单眼剥夺的影响是可逆的。1. 4只小猫在3周、4周和5周龄时右眼被闭合。所有动物在大约8周龄时进行了反向缝合，在强制使用被剥夺的眼睛2周后，对每只动物进行生理恢复测试。单细胞记录显示，最初剥夺的影响能够逆转的程度与剥夺时动物的年龄之间存在一致的关系。对一只8周龄的第五只小猫进行记录，这只小猫单眼剥夺了3周，这确保了我们最短的剥夺期会导致严重的缺陷。2. 2只小猫在单侧眼睑闭合前在黑暗中饲养了4周和5周，在8周龄时进行反向眼睑缝合时，未能显示出实质性的（大于20%）逆转。这种失败表明，在正常视觉体验后闭眼的小猫至少部分地由于其早期经历而表现出增强的可逆性，不一定是因为剥夺时间较短。其中一只小猫的同窝小猫在黑暗饲养后和眼睑缝合前有9天的正常视觉。在反向缝合6天前后进行的记录显示，眼优势几乎完全（85%）逆转。3. 3只在有过一些正常视觉体验后被单眼剥夺的动物，在双眼都保持睁开后，恢复了大量的双眼细胞。在其中一只猫身上，在睁眼之前从生理学上证实了单眼剥夺的有效性。这些发现与其他研究人员的发现形成对比，他们未能在从出生起就持续单眼剥夺、睁眼后恢复的小猫中发现许多双眼细胞。4. 1只小猫在3至5周龄时进行了2周的交替单眼剥夺饲养。在单侧眼睑再闭合4周后对这只猫进行单细胞记录，发现一些细胞抵抗了单眼剥夺，并且对被剥夺眼睛的刺激有强烈反应。对同一只动物进行反向缝合导致许多细胞（36%）恢复，这些细胞以最初被剥夺的眼睛为主导，但没有导致眼优势的逆转。
