部分肝切除大鼠血浆中的胰岛素与胰高血糖素（作者译）

[Insulin and glucagon in the blood plasma of partial hepatectomized rats (author's transl)].

作者信息

Strecker W, Silz S, Ruberstroth-Bauer G, Böttger I

出版信息

Z Naturforsch C Biosci. 1980 Jan-Feb;35(1-2):65-71.

Abstract

Up to 4 weeks after partial hepatectomy the concentrations of immunoreactive insulin and glucagon in the blood plasma of rats were determined. Furthermore we measured the activity of acid phosphatase in the serum, the concentration of cyclic AMP in liver cells, the activities of acid phosphatase in whole liver cells and in the cytosol of liver cells after partial hepatectomy. 2 h after partial hepatectomy there was a decline of the concentration of insulin in the plasma to about 13% of the initial value. 12 h after surgery a 5-fold increase of glucagon was found in the plasma. Shortly after this cyclic AMP reached its highest concentration. The activity of acid phosphatase in the whole liver cells and in the cytosol decreases slightly in the first 24 h after surgery whereas there is an increase of the activity of acid phosphatase in the serum.

摘要

在大鼠部分肝切除术后长达4周的时间内，测定了血浆中免疫反应性胰岛素和胰高血糖素的浓度。此外，我们还测量了血清中酸性磷酸酶的活性、肝细胞中环磷酸腺苷（cAMP）的浓度、部分肝切除术后全肝细胞和肝细胞胞质溶胶中酸性磷酸酶的活性。部分肝切除术后2小时，血浆中胰岛素浓度下降至初始值的约13%。手术后12小时，血浆中胰高血糖素增加了5倍。此后不久，环磷酸腺苷达到其最高浓度。全肝细胞和胞质溶胶中酸性磷酸酶的活性在手术后的头24小时略有下降，而血清中酸性磷酸酶的活性则有所增加。