Strickland S, Smith K K, Marotti K R
Cell. 1980 Sep;21(2):347-55. doi: 10.1016/0092-8674(80)90471-7.
It has previously been shown that retinoic acid induces multiple phenotypic changes in cultures of F9 teratocarcinoma stem cells. In this paper we demonstrate that these retinoid-generated cells can be converted to yet another cell type by compounds that elevate cAMP concentrations. The phenotype of the new cell type is characterized by the synthesis of plasminogen activator, laminin and type IV collagen, and by very low levels of alkaline phosphatase and lactate dehydrogenase. The secretion of plasminogen activator and type IV collagen, and low levels of alkaline phosphatase and lactate dehydrogenase, have been previously shown to be properties of parietal endoderm, an extraembryonic cell which is generated early in mouse embryonesis. We show here that parietal endoderm also synthesizes laminin. The cell type generated by retinoic acid and dibutyryl cAMP treatment is therefore indistinguishable from definitive parietal endoderm. Analysis of the final phenotype indicates that it is not dependent upon the continued presence of either compound, and that cAMP agents are active only on cells that have been treated with retinoic acid.
先前的研究表明，视黄酸可诱导F9畸胎瘤干细胞培养物发生多种表型变化。在本文中，我们证明这些类维生素A生成的细胞可被提高cAMP浓度的化合物转化为另一种细胞类型。新细胞类型的表型特征为合成纤溶酶原激活剂、层粘连蛋白和IV型胶原，且碱性磷酸酶和乳酸脱氢酶水平极低。纤溶酶原激活剂和IV型胶原的分泌以及碱性磷酸酶和乳酸脱氢酶的低水平，先前已被证明是壁内胚层的特性，壁内胚层是一种在小鼠胚胎发育早期产生的胚外细胞。我们在此表明壁内胚层也合成层粘连蛋白。因此，视黄酸和二丁酰cAMP处理产生的细胞类型与确定的壁内胚层无法区分。对最终表型的分析表明，它不依赖于这两种化合物的持续存在，且cAMP试剂仅对已用视黄酸处理的细胞有活性。
