人类嗜酸性粒细胞中过氧化物酶的特性研究。
Characterization of the peroxidase in human eosinophils.
作者信息
Wever R, Hamers M N, Weening R S, Roos D
出版信息
Eur J Biochem. 1980 Jul;108(2):491-5. doi: 10.1111/j.1432-1033.1980.tb04746.x.
Abstract
- Human eosinophils contain a peroxidase that appears to be of the same type as horseradish peroxidase, lactoperoxidase and intestine peroxidase. 2. Electron paramagnetic resonance spectra of human eosinophils show high-spin ferric heme signals with rhombic symmetry (gx = 6.56, gy = 5.31 and gx = 6.33, gy = 5.59) for the heme group. Part of the more rhombic signal is due to catalase, whereas the other part is completely due to the peroxidase. In addition to these high-spin heme compounds a low-spin heme compound is detectable with g values (gx = 3.09, gy = 2.22 and gz = 1.48) characteristic of a bisimidazole heme iron complex. 3. The amount of heme iron derived from the eosinophil peroxidase, determined from electron paramagnetic spectra, is 13.2 X 10(-17) mol/eosinophil. This is in good agreement with the pyridine hemochrome spectra which yield a value of 13.5 X 20(-17) mol heme iron/eosinophil.
摘要
- 人类嗜酸性粒细胞含有一种过氧化物酶，其似乎与辣根过氧化物酶、乳过氧化物酶和肠道过氧化物酶属于同一类型。2. 人类嗜酸性粒细胞的电子顺磁共振光谱显示，血红素基团具有菱形对称性的高自旋铁血红素信号（gx = 6.56，gy = 5.31 以及 gx = 6.33，gy = 5.59）。菱形程度更高的部分信号归因于过氧化氢酶，而另一部分则完全归因于过氧化物酶。除了这些高自旋血红素化合物外，还可检测到一种低自旋血红素化合物，其g值（gx = 3.09，gy = 2.22 和 gz = 1.48）具有双咪唑血红素铁络合物的特征。3. 根据电子顺磁共振光谱测定，源自嗜酸性粒细胞过氧化物酶的血红素铁含量为13.2×10⁻¹⁷摩尔/嗜酸性粒细胞。这与吡啶血色原光谱得出的13.5×20⁻¹⁷摩尔血红素铁/嗜酸性粒细胞的值高度一致。
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