Sakurai J, Fujii Y, Matsuura M
Microbiol Immunol. 1980;24(7):595-601. doi: 10.1111/j.1348-0421.1980.tb02862.x.
Purified beta toxin from Clostridium perfringens type C was inactivated by the oxidizing agents o-iodosobenzoate (OIBA), oxidized glutathione, and ferricyanide, and by the sulfhydryl group regents 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide, iodoacetamide, and iodoacetic acid, causing loss of activity in various degrees depending on the concentration used. The activity of the toxin was not influenced by exposure to 1.0 mM of p-chloromercuribenzoate. The toxin treated by OIBA or DTNB was reactivated by incubation with 2-mercaptoethanol and dithiothreitol. The data suggest that beta toxin contains thiol groups which are essential for the activity.
来自产气荚膜梭菌C型的纯化β毒素可被氧化剂邻碘代苯甲酸（OIBA）、氧化型谷胱甘肽和铁氰化物以及巯基试剂5,5'-二硫代双（2-硝基苯甲酸）（DTNB）、N-乙基马来酰亚胺、碘乙酰胺和碘乙酸灭活，根据所用浓度的不同，会导致不同程度的活性丧失。毒素的活性不受1.0 mM对氯汞苯甲酸暴露的影响。经OIBA或DTNB处理的毒素与2-巯基乙醇和二硫苏糖醇一起孵育可重新激活。数据表明β毒素含有对其活性至关重要的巯基。
