产气荚膜梭菌β毒素的最新研究进展
Recent insights into Clostridium perfringens beta-toxin.
作者信息
Nagahama Masahiro, Ochi Sadayuki, Oda Masataka, Miyamoto Kazuaki, Takehara Masaya, Kobayashi Keiko
机构信息
Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 770-8514, Tokushima, Japan.
Department of Microbiology, Fujita Health University School of Medicine, Toyoake 470-1192, Aichi, Japan.
出版信息
Toxins (Basel). 2015 Feb 3;7(2):396-406. doi: 10.3390/toxins7020396.
Abstract
Clostridium perfringens beta-toxin is a key mediator of necrotizing enterocolitis and enterotoxemia. It is a pore-forming toxin (PFT) that exerts cytotoxic effect. Experimental investigation using piglet and rabbit intestinal loop models and a mouse infection model apparently showed that beta-toxin is the important pathogenic factor of the organisms. The toxin caused the swelling and disruption of HL-60 cells and formed a functional pore in the lipid raft microdomains of sensitive cells. These findings represent significant progress in the characterization of the toxin with knowledge on its biological features, mechanism of action and structure-function having been accumulated. Our aims here are to review the current progresses in our comprehension of the virulence of C. perfringens type C and the character, biological feature and structure-function of beta-toxin.
摘要
产气荚膜梭菌β毒素是坏死性小肠结肠炎和肠毒血症的关键介质。它是一种形成孔道的毒素（PFT），具有细胞毒性作用。使用仔猪和兔肠袢模型以及小鼠感染模型进行的实验研究明显表明，β毒素是该生物体的重要致病因素。该毒素导致HL-60细胞肿胀和破裂，并在敏感细胞的脂筏微结构域中形成功能性孔道。随着对其生物学特性、作用机制和结构功能等方面知识的积累，这些发现代表了毒素特性研究方面的重大进展。我们在此的目的是综述目前我们对C型产气荚膜梭菌毒力以及β毒素的特性、生物学特性和结构功能的理解进展。
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