钙激活中性蛋白酶及其抑制剂：对完整肌原纤维的体外作用

Ca-activated neutral protease and its inhibitors: in vitro effect on intact myofibrils.

作者信息

Sugita H, Ishiura S, Suzuki K, Imahori K

出版信息

Muscle Nerve. 1980 Jul-Aug;3(4):335-9. doi: 10.1002/mus.880030410.

Abstract

SDS gel electrophoresis of troponin and myosin extracted from chicken myofibrils pretreated with Ca-activated neutral protease showed a pattern similar to that observed in the muscles of patients with Duchenne muscular dystrophy, that is, a decrease in troponin-I and troponin-C with relative preservation of troponin-T and degradation of the heavy chain of myosin. Also, alpha-actinin and troponin-C were released from myofibrils. Two inhibitors of Ca-activated neutral protease, leupeptin and E-64, inhibited the degradative action of Ca-activated neutral protease on intact myofibrils in vitro.

摘要

对用钙激活中性蛋白酶预处理过的鸡肌原纤维中提取的肌钙蛋白和肌球蛋白进行十二烷基硫酸钠凝胶电泳，结果显示出与杜氏肌营养不良症患者肌肉中观察到的模式相似，即肌钙蛋白I和肌钙蛋白C减少，而肌钙蛋白T相对保留，且肌球蛋白重链降解。此外，α-辅肌动蛋白和肌钙蛋白C从肌原纤维中释放出来。钙激活中性蛋白酶的两种抑制剂，亮抑酶肽和E-64，在体外抑制了钙激活中性蛋白酶对完整肌原纤维的降解作用。