河豚毒素敏感的脑细胞膜钠选择性离子载体与脂质体的关联。

The association of tetrodotoxin-sensitive, sodium-selective ionophore of brain membranes with liposomes.

作者信息

Malysheva M K, Lishko V K, Chagovetz A M

出版信息

Biochim Biophys Acta. 1980 Oct 16;602(1):70-7. doi: 10.1016/0005-2736(80)90290-4.

Abstract

The tetrodotoxin-sensitive, sodsium-selective ionophore of nerve membranes has been associated with liposomes by adding the solubilized brain microsomal fraction to a cholate/phospholipid dispersion and subsequently removing the detergent from suspension by using gel chromatography. A stimulation of the efflux of sodium from the vesicles was observed in the presence of veratrine. Tetrodotoxin itself did not effect the sodium permeability, but inhibited the veratrine-induced increment. The activation was absent in the liposomes prepared without soluble membrane proteins. The effects demonstrated for tetrodotoxin and veratrine were specific for the Na+ movement. It was possible to precipitate the tetrodotoxin-sensitive ionophore by use of (NH4)2SO4.

摘要

通过将溶解的脑微粒体部分添加到胆酸盐/磷脂分散液中，随后使用凝胶色谱法从悬浮液中去除去污剂，神经膜中对河豚毒素敏感的、对钠具有选择性的离子载体已与脂质体相关联。在藜芦碱存在的情况下，观察到钠从囊泡中的流出受到刺激。河豚毒素本身并不影响钠的通透性，但抑制了藜芦碱诱导的增加。在没有可溶性膜蛋白的情况下制备的脂质体中不存在这种激活作用。所证明的河豚毒素和藜芦碱的作用对Na + 运动具有特异性。使用硫酸铵可以沉淀对河豚毒素敏感的离子载体。