前列腺素对未成熟大鼠睾丸中鸟氨酸脱羧酶活性的影响。

Effect of prostaglandins on ornithine decarboxylase activity in the testis of immature rat.

作者信息

Madhubala R, Reddy P R

出版信息

Prostaglandins. 1980 Sep;20(3):503-13. doi: 10.1016/0090-6980(80)90038-6.

Abstract

Intratesticular injection of prostaglandin E2 (PGE2) and F2 alpha (PGF2 alpha) caused stimulation of ornithine decarboxylase (ODC) activity in the testis of immature rats. PGE2 at a dose of 10 microgram per testis was maximally effective 2 hours after the injection. Dibutyryl cyclic AMP (cAMP) and 1 methyl, 3-isobutyl xanthine (MIX), a phosphodiesterase inhibitor, also stimulated ODC activity. Simultaneous injection of PGE2 and FSH or LH caused additional stimulation of ODC activity. Similarly injection of PGE2 in addition to cAMP or MIX also caused increased stimulation of ODC. Indomethacin (IM, 60 microgram/testis) inhibited LH, FSH or cAMP induced ODC activity. However, IM at the same dose inhibited the synthesis of total proteins. These results suggest that PGE2 and PGF2 alpha stimulate the activity of ODC. The action of prostaglandins may be independent of the action of gonadotropic hormones. cAMP appears to mediate the action of prostaglandins in the testis of rat.

摘要

向未成熟大鼠睾丸内注射前列腺素E2（PGE2）和F2α（PGF2α）可刺激睾丸中鸟氨酸脱羧酶（ODC）的活性。每只睾丸注射10微克PGE2，在注射后2小时效果最佳。二丁酰环磷腺苷（cAMP）和磷酸二酯酶抑制剂1-甲基-3-异丁基黄嘌呤（MIX）也能刺激ODC活性。同时注射PGE2与促卵泡激素（FSH）或促黄体生成素（LH）可进一步刺激ODC活性。同样，除了cAMP或MIX外再注射PGE2也会增强对ODC的刺激。吲哚美辛（IM，60微克/睾丸）可抑制LH、FSH或cAMP诱导的ODC活性。然而，相同剂量的IM会抑制总蛋白的合成。这些结果表明，PGE2和PGF2α可刺激ODC的活性。前列腺素的作用可能独立于促性腺激素的作用。cAMP似乎介导了前列腺素在大鼠睾丸中的作用。