Jackson S, Lowry P J
J Endocrinol. 1980 Aug;86(2):205-19. doi: 10.1677/joe.0.0860205.
Intermediate and anterior lobes from the pituitary glands of female Wistar rats were freshly dissected and chromatographed on Sephedex G-50 and BioGel P6. Fractions were monitored with radioimmunoassays for NH2- and CO2H-terminal adrenocorticotrophin (ACTH), alpha-melanocyte-stimulating hormone (alpha-MSH), and alpha- and beta-endorphin. A large molecular weight, glycosylated form of corticotrophin-like intermediate lobe peptide (CLIP) which we have termed big CLIP, and a beta-MSH-like molecule were identified in the pars intermedia and in both lobes, a major peak of activity with the elution characteristics and cross-reactivity of gamma-lipotrophic hormone (gamma-LPH) was detected. In the pars distalis, the larger peptides 1-39 ACTH and beta-LPH predominated, whereas in the pars intermedia, the smaller peptides alpha-MSH, CLIP, beta- and alpha-endorphin were more abundant. Chromatography of rat plasma revealed peaks of immunoreactivity in the corresponding positions to those detected in the pituitary gland.
对雌性Wistar大鼠垂体的中间叶和前叶进行新鲜解剖，并在葡聚糖凝胶G-50和生物凝胶P6上进行色谱分析。通过放射免疫分析法监测各组分中的氨基端和羧基端促肾上腺皮质激素（ACTH）、α-黑素细胞刺激素（α-MSH）以及α和β内啡肽。在垂体中间叶及两个叶中鉴定出一种我们称为大CLIP的大分子量、糖基化形式的促肾上腺皮质激素样中间叶肽（CLIP）以及一种β-MSH样分子，检测到一个具有γ-促脂素（γ-LPH）洗脱特性和交叉反应性的主要活性峰。在垂体远侧部，较大的肽1-39ACTH和β-LPH占主导，而在垂体中间叶，较小的肽α-MSH、CLIP、β和α内啡肽更为丰富。大鼠血浆的色谱分析显示，其免疫反应性峰出现在与垂体中检测到的峰相对应的位置。
