Judson D G, Pay S, Bhoola K D
J Endocrinol. 1980 Oct;87(1):153-9. doi: 10.1677/joe.0.0870153.
Porcine relaxin produced a rapid, dose-related rise of cyclic AMP values in rat uterine tissue incubated in vitro. In time-course experiments, peak cyclic AMP concentrations were observed in the uterine slices at 5 min; subsequently the values fell, at first rapidly and then more slowly with the tissue concentration remaining significantly raised at 15 min. Levels of cyclic GMP in the same tissue slices were not significantly altered by relaxin. Furthermore, no increase in basal cyclic AMP values was measured in control slices prepared from the rat heart or jejunum. An increase in cyclic AMP concentration comparable to that found in the rat uterus was observed in slices of porcine uterus and cervix but not of vagina when they were stimulated with porcine relaxin. Our results suggest that the hormonal action of relaxin on the uterus and cervix is mediated through receptors linked to the enzyme, adenylate cyclase.
猪松弛素可使体外培养的大鼠子宫组织中的环磷酸腺苷（cAMP）值迅速升高，且呈剂量依赖性。在时间进程实验中，子宫切片在5分钟时观察到环磷酸腺苷浓度峰值；随后该值下降，起初下降迅速，之后下降较慢，15分钟时组织浓度仍显著升高。松弛素对同一组织切片中的环磷酸鸟苷（cGMP）水平无显著影响。此外，从大鼠心脏或空肠制备的对照切片中未检测到基础环磷酸腺苷值增加。当用猪松弛素刺激时，在猪子宫和子宫颈切片中观察到环磷酸腺苷浓度增加，与大鼠子宫中的情况相当，但阴道切片未出现这种情况。我们的结果表明，松弛素对子宫和子宫颈的激素作用是通过与酶腺苷酸环化酶相关的受体介导的。
