Wolff J, Cook G H, Goldhammer A R, Berkowitz S A
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3841-4. doi: 10.1073/pnas.77.7.3841.
The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. (i) The activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and restored by Ca2+. (ii) Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) Trifluoperazine inhibits calmodulin-activated cyclase. (iv) A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin has not been demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.
百日咳博德特氏菌的腺苷酸环化酶可被小牛脑钙调蛋白以剂量依赖的方式刺激100至1000倍。该系统具有以下特性。(i)乙二醇双(β-氨基乙醚)-N,N,N',N'-四乙酸可阻止激活,而Ca2 +可恢复激活。(ii)钙调蛋白甲硫氨酸残基的氧化消除了激活环化酶的能力。(iii)三氟拉嗪抑制钙调蛋白激活的环化酶。(iv)一种肌钙蛋白C制剂刺激百日咳博德特氏菌环化酶的效力小于钙调蛋白的0.01倍。尽管尚未在原核生物中证实存在钙调蛋白,但这是原核生物中(真核)钙调蛋白效应的一个例子。