钙调蛋白激活原核腺苷酸环化酶。
Calmodulin activates prokaryotic adenylate cyclase.
作者信息
Wolff J, Cook G H, Goldhammer A R, Berkowitz S A
出版信息
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3841-4. doi: 10.1073/pnas.77.7.3841.
Abstract
The adenylate cyclase of Bordetella pertussis is stimulated 100- to 1000-fold in a dose-dependent manner by calf brain calmodulin. The system has the following properties. (i) The activation is prevented by ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and restored by Ca2+. (ii) Oxidation of the methionine residues of calmodulin abolishes the ability to activate the cyclase. (iii) Trifluoperazine inhibits calmodulin-activated cyclase. (iv) A troponin C preparation stimulates the B. pertussis cyclase with < 0.01 the potency of calmodulin. Although calmodulin has not been demonstrated in prokaryotes, this is an example of a (eukaryotic) calmodulin effect in a prokaryote.
摘要
百日咳博德特氏菌的腺苷酸环化酶可被小牛脑钙调蛋白以剂量依赖的方式刺激100至1000倍。该系统具有以下特性。（i）乙二醇双（β-氨基乙醚）-N，N，N'，N'-四乙酸可阻止激活，而Ca2 +可恢复激活。（ii）钙调蛋白甲硫氨酸残基的氧化消除了激活环化酶的能力。（iii）三氟拉嗪抑制钙调蛋白激活的环化酶。（iv）一种肌钙蛋白C制剂刺激百日咳博德特氏菌环化酶的效力小于钙调蛋白的0.01倍。尽管尚未在原核生物中证实存在钙调蛋白，但这是原核生物中（真核）钙调蛋白效应的一个例子。
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本文引用的文献
1
Calmodulin.钙调蛋白
Annu Rev Biochem. 1980;49:489-515. doi: 10.1146/annurev.bi.49.070180.002421.
2
Calmodulin plays a pivotal role in cellular regulation.钙调蛋白在细胞调节中起关键作用。
Science. 1980 Jan 4;207(4426):19-27. doi: 10.1126/science.6243188.
3
Membrane stabilization by drugs: tranquilizers, steroids, and anesthetics.药物对膜的稳定作用：镇静剂、类固醇和麻醉剂。
Int Rev Neurobiol. 1966;9:145-221. doi: 10.1016/s0074-7742(08)60138-5.
4
A highly sensitive adenylate cyclase assay.一种高灵敏度的腺苷酸环化酶检测方法。
Anal Biochem. 1974 Apr;58(2):541-8. doi: 10.1016/0003-2697(74)90222-x.
5
Inhibition of hormone-sensitive adenyl cyclase by phenothiazines.吩噻嗪对激素敏感型腺苷酸环化酶的抑制作用。
Proc Natl Acad Sci U S A. 1970 Feb;65(2):454-9. doi: 10.1073/pnas.65.2.454.
6
Regulation of adenylate cyclase from glial tumor cells by calcium and a calcium-binding protein.钙及一种钙结合蛋白对胶质细胞瘤细胞中腺苷酸环化酶的调节作用
J Biol Chem. 1976 Aug 10;251(15):4744-50.
7
Separation and characterization of microtubule proteins from calf brain.小牛脑微管蛋白的分离与鉴定
Biochemistry. 1977 Dec 13;16(25):5610-7. doi: 10.1021/bi00644a035.
8
Rat brain adenylate cyclase. Further studies on its stimulation by a Ca2+-binding protein.大鼠脑腺苷酸环化酶。关于其受钙结合蛋白刺激的进一步研究。
Arch Biochem Biophys. 1977 Jul;182(1):124-33. doi: 10.1016/0003-9861(77)90290-9.
9
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J Biol Chem. 1977 Aug 25;252(16):5677-85.
10
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Proc Natl Acad Sci U S A. 1979 Jan;76(1):204-8. doi: 10.1073/pnas.76.1.204.
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