Auti M M
Ann Rech Vet. 1980;11(1):21-6.
Using the terminal dilution technique, a cloned virus was selected in a tissue culture systems from a heterogenous population of the original virus. The different markers studied differentiated the cloned virus from the original virus on the basis of its following characters: 1. ability to grow more efficiently at 29 than at 37 degrees C. 2. unstability of its capsid as seen by: a. high degree of sensitivity to heating at 50 degrees C for 10 min of exposure to pH 6.4 for 10 min. b. more inactivation by overnight exposure to 37 and 4 degrees C. 3. late pathogenicity in the suckling mice. The practical usefulness of a simple technique of the selection of viral clones and the possibilities of such clones to be used in the liver (modified) vaccine are discussed.
采用终点稀释技术，在组织培养系统中从原始病毒的异质群体中筛选出一种克隆病毒。所研究的不同标志物根据以下特征将克隆病毒与原始病毒区分开来：1. 在29℃比在37℃更有效地生长的能力。2. 其衣壳的不稳定性表现为：a. 在50℃加热10分钟或暴露于pH 6.4 10分钟时高度敏感。b. 在37℃和4℃过夜暴露时更易失活。3. 对乳鼠的迟发性致病性。讨论了简单的病毒克隆筛选技术的实际用途以及此类克隆用于肝脏（改良）疫苗的可能性。
