Moorman A F, de Laaf R T, Destrée O H, Telford J, Birnstiel M L
Gene. 1980 Aug;10(3):185-93. doi: 10.1016/0378-1119(80)90048-7.
Histone DNA sequences, were detected in Eco RI fragments of total Xenopus laevis DNA, by hybridization with 32P-labeled h22-DNA, a histone gene repeat unit of the sea urchin Psammechinus miliaris. The about 6 kb-size class, which was found to hybridize, was subsequently integrated into the E. coli plasmid pCR1. A clone was isolated that contains a 5.8 kb EcoRI fragment hybridizing with h22-DNA. A physical map was constructed using the restriction endonucleases BamHI, PstI, HincII, BglII, XbaI, PvuII, XhoI, AvaI, SmaI, HinfI and HpaII. The fragment was not cleaved by KpnI, AvaI, SalI and HindIII. Using this restriction map we were able to determine the gene order by hybridization with purified gene probes derived from h22-DNA. The gene order was found to be H3, H4, H2A and H2B. The localization of the H1 gene was not possible, probably due to its greater evolutionary divergence. Part of the sequence of the H3-gene is presented providing unambiguous evidence on the identity, map position and polarity of this gene.
通过与用³²P标记的h22 - DNA（海胆米氏刻肋海胆的组蛋白基因重复单元）杂交，在非洲爪蟾总DNA的Eco RI片段中检测到组蛋白DNA序列。发现约6 kb大小的类别能杂交，随后将其整合到大肠杆菌质粒pCR1中。分离出一个克隆，它含有一个与h22 - DNA杂交的5.8 kb EcoRI片段。使用限制性内切酶BamHI、PstI、HincII、BglII、XbaI、PvuII、XhoI、AvaI、SmaI、HinfI和HpaII构建了物理图谱。该片段不被KpnI、AvaI、SalI和HindIII切割。利用这个限制性图谱，我们通过与源自h22 - DNA的纯化基因探针杂交确定了基因顺序。发现基因顺序为H3、H4、H2A和H2B。由于H1基因进化差异较大，无法确定其定位。给出了H3基因的部分序列，为该基因的同一性、图谱位置和极性提供了明确证据。
