枯草芽孢杆菌温和噬菌体rho 11基因组的物理图谱。

A physical map of the genome of the Bacillus subtilis temperate phage rho 11.

作者信息

Mizukami T, Kawamura F, Takahashi H, Saito H

出版信息

Gene. 1980 Oct;11(1-2):157-62. doi: 10.1016/0378-1119(80)90095-5.

Abstract

A cleavage map of Bacillus subtilis temperate phage rho 11 was constructed with restriction endonucleases SalI, BamHI and BglII, which cut the genome into 6, 7 and 21 fragments, respectively. The molecular weight of the rho 11 genome was calculated to be 78 x 10(6). Among other endonucleases tested, PvuII, EcoRI and XbaI cleaved the genome into more than 25 fragments, while HaeIII, StuI, BalI and BamNx did not cut the genome at all. The rho 11-coded thymidylate synthetase gene, thyP11, was found to be located in the SalI-D fragment, which was in the central region of the genome.

摘要

用限制性内切酶SalI、BamHI和BglII构建了枯草芽孢杆菌温和噬菌体rho 11的切割图谱，这些酶分别将基因组切割成6个、7个和21个片段。计算得出rho 11基因组的分子量为78×10⁶。在测试的其他内切酶中，PvuII、EcoRI和XbaI将基因组切割成25个以上的片段，而HaeIII、StuI、BalI和BamNx根本不切割基因组。发现rho 11编码的胸苷酸合成酶基因thyP11位于SalI-D片段中，该片段位于基因组的中心区域。