Scher B M, Law M F, Garro A J
J Virol. 1978 Oct;28(1):395-402. doi: 10.1128/JVI.28.1.395-402.1978.
The seven previously identified EcoRI cleavage fragments of phi 105 DNA were ordered with respect to their sites of origin on the phage genome by marker rescue. One fragment, H, did not carry any determinants essential for replication. This fragment was totally missing in a deletion mutant which exhibited a lysogenization-defective phenotype. There is a nonessential region on the phi 105 genome which begins in fragment B, spans fragment H, and ends in fragment F. The size of the nonessential region, as estimated by alterations observed in the fragmentation patterns of deletion mutant DNAs, is approximately 2.7 X 10(6) daltons. Two new EcoRI cleavage fragments with molecular weights of approximately 0.2 X 10(6) were detected by autoradiography of 32P-labeled DNA. These small fragments were not located on the cleavage map.
通过标记拯救，按照噬菌体基因组上的起源位点对先前鉴定出的噬菌体φ105 DNA的7个EcoRI切割片段进行了排序。其中一个片段H不携带复制所必需的任何决定簇。在一个表现出溶原化缺陷表型的缺失突变体中，这个片段完全缺失。噬菌体φ105基因组上有一个非必需区域，它始于片段B，跨越片段H，止于片段F。根据缺失突变体DNA片段化模式中观察到的变化估计，该非必需区域的大小约为2.7×10⁶道尔顿。通过对³²P标记的DNA进行放射自显影，检测到两个分子量约为0.2×10⁶的新EcoRI切割片段。这些小片段不在切割图谱上。
