Butcher R G, Dawson A L, Knaab S A, Gahan P B
Histochem J. 1980 Sep;12(5):591-8. doi: 10.1007/BF01011932.
Formazans can be lost from tissue sections when the incubation medium is removed at the end of a dehydrogenase reaction. The loss is not uniform and will clearly affect the interpretation of results. This suggests that both the qualitative evaluation of the reaction and the measurement of enzyme activity should be made at the time of incubation.
在脱氢酶反应结束时去除孵育介质时,组织切片中的甲臜可能会丢失。这种丢失并不均匀,显然会影响结果的解读。这表明反应的定性评估和酶活性的测量都应在孵育时进行。