噬菌体T4和藤黄微球菌紫外线特异性内切核酸酶对嘧啶二聚体切割作用的比较。

Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases.

作者信息

Gordon L K, Haseltine W A

出版信息

J Biol Chem. 1980 Dec 25;255(24):12047-50.

Abstract

A comparison was made of the activity of the UV-specific endonucleases of bacteriophage T4 (T4 endonuclease V) and of Micrococcus luteus on ultravilet light-irradiated DNA substrates of defined sequence. The two enzymes cleave DNA at the site of pyrimidine dimers with the same frequency. The products of the cleavage reaction are the same, suggesting that the scission of DNA by T4 endonuclease V occurs via the combined actin of a pyrimidine dimer specific DNA glycosylase and an apyrimidinic-apurinic (AP) endonuclease as was recently shown for the M. luteus enzyme. The pyrimidine dimer DNA-glycosylase activity of both enzymes is more active on double-stranded DNA than it is on single-stranded DNA.

摘要

对噬菌体T4（T4内切核酸酶V）和藤黄微球菌的紫外线特异性内切核酸酶在特定序列的紫外线照射DNA底物上的活性进行了比较。这两种酶在嘧啶二聚体位点切割DNA的频率相同。切割反应的产物相同，这表明T4内切核酸酶V切割DNA是通过嘧啶二聚体特异性DNA糖基化酶和脱嘧啶-脱嘌呤（AP）内切核酸酶的联合作用，正如最近对藤黄微球菌酶所显示的那样。两种酶的嘧啶二聚体DNA糖基化酶活性在双链DNA上比在单链DNA上更活跃。