Hollins C, Stone T W, Lloyd H
Neurosci Lett. 1980 Nov;20(2):217-21. doi: 10.1016/0304-3940(80)90149-4.
Slices of mouse or rat cerebral cortex were incubated with [3H]adenine or [3H]adenosine, and [14C]GABA. Purines and GABA could subsequently be released by ouabain. The release of purines previously shown to occur on restoring elevated K+ levels to normal was not mimicked by noradrenaline at concentrations which activate (Na+,K+)-ATPase. Potassium-free solutions evoked no release of purine during the test period, but resulted in a large release when K+ was restored to normal. K+-free solutions evoked an immediate release of GABA. It is concluded that (Na+,K+)-ATPase is not involved in purine release.
将小鼠或大鼠的大脑皮层切片与[3H]腺嘌呤或[3H]腺苷以及[14C]γ-氨基丁酸（GABA）一起孵育。随后，哇巴因可使嘌呤和GABA释放。去甲肾上腺素在激活（Na +，K +）-ATP酶的浓度下，无法模拟先前在将升高的钾离子水平恢复正常时所发生的嘌呤释放。在测试期间，无钾溶液不会引起嘌呤释放，但当钾离子恢复正常时会导致大量释放。无钾溶液会立即引起GABA释放。得出的结论是，（Na +，K +）-ATP酶不参与嘌呤释放。
