Tsou K C, Yip K F
Nucleic Acids Res. 1980 Feb 11;8(3):567-72. doi: 10.1093/nar/8.3.567.
Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-2-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phosphodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.
用核糖核酸酶N1可以很容易地合成1,N6-乙烯基腺苷-2-磺酸盐或1,N6-乙烯基-2-氮杂腺苷的3'-磷酸鸟苷酯。磷酸二酯和起始荧光核苷之间的量子产率有10倍的差异。这种差异为开发一种用于核酸酶的荧光测定法提供了基础。
