Kopito R R, Brunengraber H
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5738-40. doi: 10.1073/pnas.77.10.5738.
(R)-Mevalonate was identified by radioenzymatic assay in human and rat urines. This was confirmed by spectrophotometric enzymatic assay of an ether extract of human urine. The average excretion rate of (R)-mevalonate in humans was 1.7 mumol/24 hr which corresponds to 29% of the glomerular filtration rate. In anesthetized rats the average rate of (R)-mevalonate excretion was 350 pmol/min, corresponding to 44% of the glomerular filtration rate. These rates were not affected by the sex of the subjects or animals. After bilateral nephrectomy, the concentration of (R)-mevalonate in rat serum increased within 2 hr to a new level that was 5 times that of sham-operated controls. Nephrectomized rats showed a decrease in their tolerance to an intravenous load of mevalonate. These data show that (R)-mevalonate is normally excreted in the urine of humans and rats. Urinary excretion of (R)-mevalonate should therefore be taken into account in in vivo studies on the metabolism of this compound.
通过放射酶法在人和大鼠尿液中鉴定出了（R）-甲羟戊酸。这通过对人尿乙醚提取物的分光光度酶法得以证实。人类中（R）-甲羟戊酸的平均排泄率为1.7微摩尔/24小时，相当于肾小球滤过率的29%。在麻醉大鼠中，（R）-甲羟戊酸的平均排泄率为350皮摩尔/分钟，相当于肾小球滤过率的44%。这些排泄率不受受试者或动物性别的影响。双侧肾切除术后，大鼠血清中（R）-甲羟戊酸的浓度在2小时内升高至新水平，是假手术对照组的5倍。肾切除大鼠对静脉注射甲羟戊酸负荷的耐受性降低。这些数据表明，（R）-甲羟戊酸通常在人和大鼠尿液中排泄。因此，在关于该化合物代谢的体内研究中应考虑（R）-甲羟戊酸的尿排泄情况。
