噬菌体λ Sam 7 h 22的组蛋白DNA序列在质粒pBR 322中的亚克隆。

Subcloning of the histone DNA sequences of phage lambda Sam 7 h 22 in plasmid pBR 322.

作者信息

Aulehla-Scholz C, Jacob E

出版信息

Z Naturforsch C Biosci. 1980 Sep-Oct;35(9-10):733-40. doi: 10.1515/znc-1980-9-1014.

DOI:10.1515/znc-1980-9-1014

Abstract

The histone DNA sequences of the Hind III cluster of the sea urchin Psammechinus miliaris which are carried by the chimeric phage DNA of lambda Sam 7 h 22 have been subcloned in plasmid p BR 322. Due to this procedure single segments of the cluster have been separated from each other and are available as gene specific hybridization probes.

摘要

由λSam 7 h 22的嵌合噬菌体DNA携带的海胆米氏刻肋海胆（Psammechinus miliaris）Hind III簇的组蛋白DNA序列已被亚克隆到质粒p BR 322中。通过这个过程，该簇的单个片段已相互分离，并可作为基因特异性杂交探针使用。