一个类似转座子的DNA片段中断了多头绒泡菌组蛋白H4基因。
A transposon-like DNA fragment interrupts a Physarum polycephalum histone H4 gene.
作者信息
Wilhelm M L, Wilhelm F X
出版信息
FEBS Lett. 1984 Mar 26;168(2):249-54. doi: 10.1016/0014-5793(84)80256-2.
PMID:6327366
Abstract
A recombinant DNA library was screened for histone H4 genes using a sea urchin probe. One recombinant was analysed by restriction enzyme mapping and Southern blotting. The complete DNA sequence of the H4 histone locus was determined. An 86 base pair interrupting sequence was found within the histone H4 coding sequence. The inserted DNA fragment has some characteristics of a transposable element.
摘要
使用海胆探针筛选重组DNA文库以寻找组蛋白H4基因。通过限制性酶切图谱分析和Southern印迹法对一个重组体进行了分析。确定了H4组蛋白基因座的完整DNA序列。在组蛋白H4编码序列中发现了一个86个碱基对的中断序列。插入的DNA片段具有一些转座元件的特征。
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