Tchao R, Leighton J
Cancer Res. 1981 Feb;41(2):635-9.
Ten cases of human urinary bladder tumors, varying from histological Grades I to III, were cultured as fragments on a gyratory shaker. Low-grade tumors formed cysts spontaneously, and dibutyryl cyclic adenosine 3':5'-monophosphate stimulated both the number and the size of cysts. Grade III tumors did not form any cysts in culture, and, with light microscopy, dibutyryl cyclic adenosine 3':5'-monophosphate had no visible effect on the fragments. The cells lining the cysts that occurred in the aggregates were characterized by the appearance of zonula occludens on the aspect of the cells facing the culture medium. Two types of cysts were observed: some were lined by a monolayer of cells; and some were lined by a multilayer. In the former, zonula occludens appeared on the aspect facing the culture medium; in the latter, zonula occludens appeared on both the aspect facing the culture medium and that facing the cavities. These specialized structures functioned as tight intercellular junctions as one component of the complex of functions required for effective transport by sheets of epithelium.
十例组织学分级为I至III级的人类膀胱肿瘤，被切成碎片在旋转振荡器上培养。低级别肿瘤能自发形成囊肿，二丁酰环磷腺苷可刺激囊肿的数量和大小。III级肿瘤在培养中未形成任何囊肿，并且在光学显微镜下，二丁酰环磷腺苷对肿瘤碎片没有明显影响。聚集物中出现的囊肿内衬细胞的特征是，在细胞面向培养基的一侧出现紧密连接。观察到两种类型的囊肿：一些由单层细胞衬里；一些由多层细胞衬里。在前者中，紧密连接出现在面向培养基的一侧；在后者中，紧密连接出现在面向培养基的一侧和面向腔的一侧。这些特殊结构作为紧密的细胞间连接发挥作用，是上皮细胞片有效运输所需复杂功能的一个组成部分。
