Berlan M, Lafontan M
Eur J Pharmacol. 1980 Oct 31;67(4):481-4. doi: 10.1016/0014-2999(80)90191-0.
3H-clonidine bound to membrane sites of human fat cells, which have the characteristics of alpha 2-adrenoceptors. Specific binding was rapid, reversible and saturable. [3H]Clonidine binding was of high affinity with a KD of 3.9 nM and with a maximal occupancy of 348 fmol/mg protein. The correlation between alpha-adrenergic agonist or antagonist affinities for the membrane [3H]clonidine binding site with their physiological potencies demonstrates the usefulness of the human fat cell as a model for investigating postsynaptic alpha 2-adrenoceptor properties and regulation.
3H-可乐定与人脂肪细胞膜位点结合，这些位点具有α2-肾上腺素能受体的特征。特异性结合迅速、可逆且具有饱和性。[3H]可乐定结合具有高亲和力，解离常数（KD）为3.9 nM，最大结合量为348 fmol/mg蛋白质。α-肾上腺素能激动剂或拮抗剂对膜[3H]可乐定结合位点的亲和力与其生理效能之间的相关性表明，人脂肪细胞作为研究突触后α2-肾上腺素能受体特性和调节的模型具有实用性。
